Evaluation of the direct agglutination test (DAT) using freeze-dried antigen for the detection of anti-Leishmania antibodies in stored sera from various patient groups in Ethiopia
L. Oskam et al., Evaluation of the direct agglutination test (DAT) using freeze-dried antigen for the detection of anti-Leishmania antibodies in stored sera from various patient groups in Ethiopia, T RS TROP M, 93(3), 1999, pp. 275-277
Citations number
16
Categorie Soggetti
Medical Research General Topics
Journal title
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
Tests on a panel of 421 serum samples from Ethiopia and 11 from Europe with
the direct agglutination test (DAT) and freeze-dried antigen (Leishmania d
onovani 1S) showed that a cut-off titre of 1:200 or 1:400 could be employed
. Using a cut-off titre of 1:400, 181 (88.3%) of 205 serum samples from vis
ceral leishmaniasis (VL) patients tested positive, as did 5 (13.9%) of 36 s
era from patients with localized cutaneous leishmaniasis, 5 (71.4%) sera fr
om 7 patients with diffuse cutaneous leishmaniasis and 8 (89.9%) sera from
9 HIV-positive VL patients. We also found that 19 (57.6%) sera from 33 trea
ted VL patients were still positive in the DAT at 1.5 to 5 years after trea
tment.