Size determination of bacterial capsular oligosaccharides used to prepare conjugate vaccines

Citation
N. Ravenscroft et al., Size determination of bacterial capsular oligosaccharides used to prepare conjugate vaccines, VACCINE, 17(22), 1999, pp. 2802-2816
Citations number
27
Categorie Soggetti
Veterinary Medicine/Animal Health",Immunology
Journal title
VACCINE
ISSN journal
0264410X → ACNP
Volume
17
Issue
22
Year of publication
1999
Pages
2802 - 2816
Database
ISI
SICI code
0264-410X(19990716)17:22<2802:SDOBCO>2.0.ZU;2-H
Abstract
We recently described the use of ion exchange chromatography for analysis a nd the industrial scale preparation of pools of oligsaccharides of intermed iate chain length from polysaccharides of Haemophilus influenzae of type b (Hib) and Neisseria meningitidis groups A and C. These negatively charged " sized" oligosaccharides are activated and conjugated to the carrier protein (CRM197) to prepare the corresponding glycoconjugate vaccines. Characteriz ation and accurate determination of the degree of polymerization (DP) of th e pool of oligosaccharides is essential for the consistent production of th ese conjugate vaccines. This paper describes the colorimetric assays used f or determination of the average DP of the Hib and meningococcal oligosaccha rides, and the qualification of these assays achieved by size characterizat ion of the respective oligosaccharides by use of physicochemical methods, i ncluding liquid chromatography. mass spectrometry (ionspray) and NMR spectr oscopy. (C) 1999 Elsevier Science Ltd. All rights reserved.