Hgp. Van Gennip et al., An internal duplication in the 5 ' noncoding region of strain H: A bovine viral diarrhoea virus (BVDV) isolated from pigs, VIRUS GENES, 19(1), 1999, pp. 45-49
A pig pestivirus isolate, strain H, was characterized by using reverse tran
scription-PCR (RT-PCR) and direct sequencing of the amplicons. A duplicatio
n of 74 nucleotides was found at the 5' terminus of the 5' noncoding (NC) r
egion, which was also found in RNA isolates from tonsils from two other pig
s from the same farm. When the duplication was omitted, the 5' NC region sh
owed 97.8% similarity to bovine viral diarrhoea virus (BVDV) strain Korevaa
r and 94% to BVDV strain Osloss. Furthermore, the rearrangement of the 5' N
C region of strain H was maintained after passaging in different cell lines
and is not common for ruminant-like pestivirus isolated from pigs. Phyloge
netic analysis based on the deduced amino acid sequence of the E2 gene of s
train H confirmed the findings of the 5' NC region and show that this strai
n belongs to the BVDVIb subgroup. These results show for the first time rea
rrangements in the 5' NC region of a pestivirus.