An internal duplication in the 5 ' noncoding region of strain H: A bovine viral diarrhoea virus (BVDV) isolated from pigs

Citation
Hgp. Van Gennip et al., An internal duplication in the 5 ' noncoding region of strain H: A bovine viral diarrhoea virus (BVDV) isolated from pigs, VIRUS GENES, 19(1), 1999, pp. 45-49
Citations number
23
Categorie Soggetti
Molecular Biology & Genetics
Journal title
VIRUS GENES
ISSN journal
09208569 → ACNP
Volume
19
Issue
1
Year of publication
1999
Pages
45 - 49
Database
ISI
SICI code
0920-8569(199907)19:1<45:AIDIT5>2.0.ZU;2-A
Abstract
A pig pestivirus isolate, strain H, was characterized by using reverse tran scription-PCR (RT-PCR) and direct sequencing of the amplicons. A duplicatio n of 74 nucleotides was found at the 5' terminus of the 5' noncoding (NC) r egion, which was also found in RNA isolates from tonsils from two other pig s from the same farm. When the duplication was omitted, the 5' NC region sh owed 97.8% similarity to bovine viral diarrhoea virus (BVDV) strain Korevaa r and 94% to BVDV strain Osloss. Furthermore, the rearrangement of the 5' N C region of strain H was maintained after passaging in different cell lines and is not common for ruminant-like pestivirus isolated from pigs. Phyloge netic analysis based on the deduced amino acid sequence of the E2 gene of s train H confirmed the findings of the 5' NC region and show that this strai n belongs to the BVDVIb subgroup. These results show for the first time rea rrangements in the 5' NC region of a pestivirus.