Propolis from Central Chile was investigated for its plant origin by micros
copical analysis of pollen grains and leaf fragments found in the sample. T
he pollen grains that appear with significant higher frequency in the sampl
e corresponded to four native and two introduced species, whereas lear frag
ments corresponded to four native species. Seventeen phenolic compounds tha
t belong to the phenylpropane, benzaldehyde, dihydrobenzofuran, or benzopyr
an classes, were isolated from an organic extract that was found to have a
moderate growth inhibitory activity against Mycobacterium avium, M. tubercu
losis, and two strains of Staphylococcus aureus. The components responsible
for activity were determined.