Activin-treated urodele animal caps: II. Inductive interactions in newt animal caps after treatment with activin A

The inductive interactions between activin-induced and non-induced cells we re investigated in newt animal cap explants. A wide range of concentrations of activin A (0.1-100 ng/ml) induced mesodermal tissues in the animal caps , but at generally low frequencies. Animal caps treated with 100 ng/ml of a ctivin A, on the other hand, differentiated solely into nonspecific endoder m. At this concentration, various mesodermal tissues were induced in additi on to endoderm as the animal caps increased in size. They were more frequen tly induced in explants in which the activin-treated animal caps were combi ned with untreated animal caps. Central nervous systems were frequently ind uced in sandwich explants with larger amounts of untreated animal caps. Dif ferentiation of endodermal organs such as the liver, the pancreas and the i ntestine in the long-term cultured sandwich explants was confirmed by elect ron microscopy. Lineage tracing of the combination and sandwich explants re vealed that the activin-treated animal caps mainly formed the endoderm and induced mesodermal and neural tissues in the untreated animal caps. These r esults suggest that activin A is capable of inducing the endoderm that can act as an initiator of further inductive interactions in early newt develop ment.