Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain

The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concent rated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All cry stals are isomorphous and belong to space group P2(1)2(1)2(1). The unit-cel l dimensions for wild-type R2 crystals grown from sodium citrate precipitan t are a = 28.83, b = 40.18, c = 49.23 Angstrom. Crystals contain one R2 mol ecule per asymmetric unit. They are stable during 3 d exposure to X-rays an d diffract to 1.37-1.45 Angstrom resolution.