Norepinephrine-induced Ca2+ waves depend on InsP(3) and ryanodine receptoractivation in vascular myocytes

In rat portal vein myocytes, Ca2+ signals can be generated by inositol 1,4, 5-trisphosphate (InsP(3))- and ryanodine-sensitive Ca2+ release channels, w hich are located on the same intracellular store. Using a laser scanning co nfocal microscope associated with the patch-clamp technique, we showed that propagated Ca2+ waves evoked by norepinephrine (in the continuous presence of oxodipine) were completely blocked after internal application of an ant i-InsP(3) receptor antibody. These propagated Ca2+ waves were also reduced by similar to 50% and transformed in homogenous Ca2+ responses after applic ation of an antiryanodine receptor antibody or ryanodine. All-or-none Ca2waves obtained with increasing concentrations of norepinephrine were transf ormed in a dose-response relationship with a Hill coefficient close to unit y after ryanodine receptor inhibition. Similar effects of the ryanodine rec eptor inhibition were observed on the norepinephrine- and ACh-induced Ca2responses in non-voltage-clamped portal vein and duodenal myocytes and on t he norepinephrine-induced contraction. Taken together, these results show t hat ryanodine-sensitive Ca2+ release channels are responsible for the fast propagation of Ca2+ responses evoked by various neurotransmitters producing InsP(3) in vascular and visceral myocytes.