Ae. Lyubarev et Bi. Kurganov, Modeling of irreversible thermal protein denaturation at varying temperature. II. The complete kinetic model of Lumry and Eyring, BIOCHEM-MOS, 64(7), 1999, pp. 832-838
The moder for thermal denaturation of proteins involving consecutive revers
ible and irreversible steps (Lumry and Eyring model) has been analyzed. The
most general case, when equilibrium in the first step is established slowl
y in comparison with the rate of the second step and the heat effect value
for the second step is either greater than or less than zero, has been cons
idered. The theoretical dependences of excess heat; capacity on temperature
have been constructed. The variation of the shape of the theoretical curve
s with varied values of the enthalpy change for the second step, Arrhenius
equation parameters for both steps, and the scanning rate has been studied.