Nucleotide sequence, heterologous expression and novel purification of DNAligase from Bacillus stearothermophilus

Authors
Brannigan, JA Ashford, SR Doherty, AJ Timson, DJ Wigley, DB
Citation
Ja. Brannigan et al., Nucleotide sequence, heterologous expression and novel purification of DNAligase from Bacillus stearothermophilus, BBA-PROT ST, 1432(2), 1999, pp. 413-418
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
ISSN journal
01674838 → ACNP
Volume
1432
Issue
2
Year of publication
1999
Pages
413 - 418
Database
ISI
SICI code
0167-4838(19990713)1432:2<413:NSHEAN>2.0.ZU;2-Z
Abstract
The gene for DNA ligase (EC 6.5.1.2) from thermophilic bacterium Bacillus s tearothermophilus NCA1503 has been cloned and the complete nucleotide seque nce determined. The ligase gene encodes a protein 670 amino acids in length . The gene was overexpressed in Escherichia coli and the enzyme has been pu rified to homogeneity. Preliminary characterisation confirms that it is a t hermostable, NAD(+)-dependent DNA ligase. (C) 1999 Elsevier Science B.V. Al l rights reserved.