Several epidemiological studies have indicated that female tobacco smokers
may be at higher risk of lung cancer than males, In a study of lung cancer
cases, we have found that female smokers had a significantly higher level o
f aromatic/hydrophobic DNA adducts in their nontumor lung tissue (15.39 +/-
9.47 adducts/10(8) nucleotides, n = 29) than male smokers (12.08 +/- 8.14,
n = 93; P = 0.047). Females had significantly higher levels of adducts/pac
k-year (females 0.95 +/- 0.82 adducts/pack-year and males 0.46 +/- 0.46; P
= 0.0004) and adducts/cigarette/day (females 1.48 +/- 1.29 and males 0.89 /- 0.74, P = 0.015). By quantitative reverse transcription-PCR, it was foun
d that female smokers exhibited a significantly higher expression Level of
Lung CYP1A1 (494 +/- 334 CYP1A1 mRNA/10(6) glyceraldehyde-3-phophate dehydr
ogenase mRNA, n = 15) compared with males (210 +/- 208, n = 12; P = 0.016).
Furthermore, for both sexes combined a significant correlation between CYP
1A1 expression and DNA adduct level was found (r = 0.50, P = 0.009), In con
clusion, the observed sex difference in aromatic/hydrophobic DNA adduct lev
els may at least in part be explained by different Levels of CYP1A1 express
ion.