Nickel(II) increases the sensitivity of V79 Chinese hamster cells towards cisplatin and transplatin by interference with distinct steps of DNA repair

Citation
I. Krueger et al., Nickel(II) increases the sensitivity of V79 Chinese hamster cells towards cisplatin and transplatin by interference with distinct steps of DNA repair, CARCINOGENE, 20(7), 1999, pp. 1177-1184
Citations number
41
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CARCINOGENESIS
ISSN journal
01433334 → ACNP
Volume
20
Issue
7
Year of publication
1999
Pages
1177 - 1184
Database
ISI
SICI code
0143-3334(199907)20:7<1177:NITSOV>2.0.ZU;2-A
Abstract
Nickel compounds are carcinogenic to humans and to experimental animals. In contrast to their weak mutagenicity; they have been shown previously to in crease UV-induced cytotoxicity and mutagenicity and to interfere with the r epair of UV-induced DNA lesions by disrupting DNA-protein interactions invo lved in DNA damage recognition. In the present study me applied cisplatin, transplatin and mitomycin C to investigate whether these enhancing effects and DNA repair inhibition are also relevant for other DNA damaging agents, Nickel(II) at non-cytotoxic concentrations of 50 mu M and higher caused a p ronounced increase in cisplatin-, transplatin- and mitomycin C-induced cyto toxicity, which was neither due to an altered uptake of cisplatin or transp latin nor to an increase in DNA adduct formation, However, nickel(II) inhib ited the repair of cisplatin- and transplatin-induced DNA lesions, In combi nation with transplatin, it decreased the incision frequency, indicating th at the DNA damage recognition/incision step during nucleotide excision repa ir is affected in general by nickel(II), In support of this, concentrations as low as 10 mu M nickel(II) decreased binding of the xeroderma pigmentosu m complementation group A protein to a cisplatin-damaged oligonucleotide, W hen combined with cisplatin, the incision frequency was affected only margi nally, while nickel(II) led to a marked accumulation of DNA strand breaks, indicating an inhibition of the polymerization/ligation step of the repair process. This effect may be explained by interference with the repair of DN A-DNA interstrand crosslinks induced by cisplatin. Our results suggest that nickel(II) at non-cytotosic concentrations inhibits nucleotide excision re pair and possibly crosslink repair by interference with distinct steps of t he respective repair pathways.