A. Pielesz et al., Separation of aromatic amines of MAK group, which are the reduction products of AZO dyes by partition HPLC chromatography, CHEM ANAL, 44(3B), 1999, pp. 495-504
HPLC chromatographic systems allowing the separation and the determination
of aromatic amines of MAK group, whose presence in textile products is proh
ibited, have been worked out. 29 aromatic amines were investigated. The app
lication of C-18 columns and gradient of methanol-water [45 + 64 v/v for 12
min to 70 + 30 for 8 min and 80 + 20 v/v for 5 min to 85 + 15 for 10 min]
with flow rate of 1 ml min(-1) allowed to separate and determine 17 of test
ed amines such as: 1,3-phenyldiamine, 2,4-diaminotoluene, 1,4-phenyldiamine
, aniline, 4,4'-diaminediphenyl ether, o-toluidine, p.-toluidine, 4,4'-diam
inodiphenylmethane, 2-amino-4-nitrotoluene, o-dianisidine, o-tolidin, aniso
le, 4-chloro-2-methylaniline, 2,4,6-trimethylaniline, 3,3'-dichlorobenzidin
e, diphenylamine, o-amino-azotoluene. Use of the mixture of methanol-water
53-47 v/v and flow rate 1.2 ml min(-1) allows to determine simultaneously 1
,2-phenyldiamine, 4,4'-thiodianiline, benzidine, 1-naphtylamine, m-toluidin
e, 4-methoxy-1,3-phenylenediamine sulfate, 4,4'-diamino-3,3'-dimethyldiphen
ylmethane, 4,4'-methylene-bis(2-chloroaniline), 2,4,5-trimethylaniline. Mea
surements were performed with the use, of DAD spectrophotometric detector a
nd fluorescence detector. Moreover, the identification of analyzed aromatic
amines in samples of the following dyes was carried out: direct orange G.
direct red 4B, direct blue 2B, folan red G, D Brown 95, D Blue 1 and colour
ed yam after reduction with artificial sweat.