Biosphere 2 is a 1.27 ha virtually airtight environmental research facility
located in Oracle, AZ. In this paper we emphasize material selection, supe
r-structure, and sealing and testing methods to maintain a materially close
d environment with a leak rate of approximate to 10% volumetric exchange pe
r year, a specification some 1000 times the commercial building industry st
andard. The capability to sustain closure was demonstrated over a period of
similar to 3 years with human inhabitants reflecting one of the most uniqu
e and sophisticated structural features of the facility distinguishing it f
rom large greenhouses and phytotrons. A brief introduction to the diverse s
tructures of the enclosed mesocosms is given including the wilderness and h
uman-occupied areas. Key mechanical and electrical systems are summarized a
nd recent modifications affecting the operation of Biosphere 2 as a closed
system are described. (C) 1999 Elsevier Science B.V. All rights reserved.