A study of the electrochemistry of carnosine based on the coupling reaction with diazo-p-amino acetophenone

Camosine could be determined in the range between 1.0 x 10(-7) and 5.5 x 10 (-6) M by differential pulse adsorptive stripping voltammetry (DPASV) on a static mercury electrode after derivatization with diazo-p-aminoacetophenon e (DPAAP) at pH 9.6-9.7. The detection limit was 6.8 x 10(-8) M, the relati ve standard deviation (n = 10) for 5.0 x 10(-6) M of carnosine was 1.8%. Th e coupling reaction and electrochemical reaction mechanism of azo-carnosine were investigated by structure analysis, UV spectroscopic, cyclic voltamme try, DPASV and electrocapillary at mercury electrodes. A reversible two-ele ctron via two-proton redox and adsorption-controlled process occurred in fo ur consecutive steps with an intermediate at the mercury electrode.