Detection of nitric oxide with the iron (III) porphyrin doped nafion/glassy carbon electrode

Nitric oxide dissolved in buffer solutions has been detected by the electro chemical oxidation reaction on a glassy carbon (GC) electrode coated with N afion film doped with various iron (III) porphyrins. The electrochemical de tection was carried out by using different methods, such as normal pulse vo ltammetry (NPV), differential pulse voltammetry (DPV) and amperometry. All iron (III) porphyrins examined showed a catalytic activity for the electrol ytic oxidation of nitric oxide. In the result, tetrakis-(pentafluorophengl) porphyrin iron (III) chloride (Fe(TPFPP)CI) showed the most prominent elec trocatalytic activity. Amperometric detection of nitric oxide was successfu lly performed on the GC electrode coated with a Nafion film doped with Fe(T PFPP)CI. In the amperometry with an injection of NO solution, the lower det ection limit was found to be about 10 nM.