I. Manduteanu et al., High glucose induces enhanced monocyte adhesion to valvular endothelial cells via a mechanism involving ICAM-1, VCAM-1 and CD18, ENDOTHELIU, 6(4), 1999, pp. 315-324
Upon induction of experimental hyperglycemia (i.e. diabetes) pathological m
odifications are early detected (similar to 7 days) at the level of the car
diac valves leading rapidly to the development of valvular atheroma. Monocy
te adhesion to the Vascular endothelium is one of the initial event at the
onset of atherosclerosis. We questioned whether high glucose enhances monoc
yte adhesion to the valvular endothelial cells (VEC) so as to explain, in p
art, the accelerated atheroma formation that occur in diabetic conditions.
To this purpose we compared the adhesion of monocytes to VEC cultured in 5.
5 mM (normal) glucose (NG) or in 33mM (high) glucose (HG) or in high mannit
ol (HM) (27.5 mM mannitol plus 5.5 mM glucose), a concentration known to si
mulate the hyperosmolar effect of high glucose. After incubation for 30 min
at 37 degrees C, the adhesion of monocyte cell line (U937 cells) to VEC wa
s quantitated by a fluorimetric assay or by direct counting. Statistical da
ta showed a significant increased adhesion of monocytes to VEC grown in HG
(up to 4 fold) or in HM (up to 2.7) when compared to normal conditions. Usi
ng a battery of specific monoclonal antibodies molecules it was found that
the increased adhesion of monocytes to VEC grown in high glucose was specif
ically inhibited (p < 0.05) by anti-ICAM-1, anti-VCAM-1 and anti-CD18 monoc
lonal antibodies. Together, the results indicate that high glucose induces
enhanced monocyte adhesion to VEC via a mechanism involving in part an osmo
tic effect and mainly the cell adhesion molecules: ICAM-1, VCAM-1 and CD18.