Fv. Pereirameirelles et al., A STABLE LIPASE FROM CANDIDA-LIPOLYTICA - CULTIVATION CONDITIONS AND CRUDE ENZYME CHARACTERISTICS, Applied biochemistry and biotechnology, 63-5, 1997, pp. 73-85
Although lipases have been intensively studied, some aspects of enzyme
production Like substrate uptake, catabolite repression, and enzyme s
tability under long storage periods are seldom discussed in the Litera
ture. This work deals with the production of lipase by a new selected
strain of Candida lipolytica. Concerning nutrition, it was observed th
at inorganic nitrogen sources were not as effective as peptone, and th
at oleic acid or triacylglycerides (TAG) were essential carbon sources
. Repression by glucose and stimulation by oleic acid and long chain T
AG (triolein and olive oil) were observed. Extracellular Lipase activi
ty was only observed at high levels at late stationary phase, whereas
intracellular lipase levels were constant and almost undetectable duri
ng the cultivation period, suggesting that the produced enzyme was att
ached to the cell wall, mainly at the beginning of cultivation. The cr
ude lipase produced by this yeast strain shows the following optima co
nditions: pH 8.0-10.0, temperature of 55 degrees C. Moreover, this pre
paration maintains its full activity for at least 370 d at 5 degrees C
.