Establishment of an embryonic stem (ES) cell line derived from a non-obesediabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission

Authors
Nagafuchi, S Katsuta, H Kogawa, K Akashi, T Kondo, S Sakai, Y Tsukiyama, T Kitamura, D Niho, Y Watanabe, T
Citation
S. Nagafuchi et al., Establishment of an embryonic stem (ES) cell line derived from a non-obesediabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission, FEBS LETTER, 455(1-2), 1999, pp. 101-104
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
455
Issue
1-2
Year of publication
1999
Pages
101 - 104
Database
ISI
SICI code
0014-5793(19990716)455:1-2<101:EOAES(>2.0.ZU;2-M
Abstract
A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic E S cell-like morphology, expressing the stem cell marker alkaline phosphatas e, and displaying a normal diploid karyotype, After injecting the NODES cel ls into blastocysts, chimeric mice were obtained. Small but significant num bers of lymphocytes expressed the NOD-derived MHC allele, When a chimeric m ouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(beta)(g7) haplotype. Thus, an NOD-ES cell line which ca o differentiate into lymphocytes with potential for germ line transmission was successfully established. (C) 1999 Federation of European Biochemical S ocieties.