Comparative allelotyping of the short arm of human chromosome 3 in epithelial tumors of four different types

Comparative allelotyping of the short arm of human chromosome 3 (3p) in fou r types of epithelial carcinomas was performed using an identical set of po lymorphic markers. In total, 117 samples of non-papillary renal cell carcin oma (RCC), non-small cell lung carcinoma (NSCLC), carcinoma of uterine cerv ix (CC), and breast carcinoma (BC) were screened for loss of heterozygosity (LOH) with 10 di-, tri- and tetrameric markers covering nine bands of 3p. High LOH frequencies were detected in at least one locus: RCC (36/43, 84%), BC (20/26, 77%), NSCLC (16/24, 67%), and CC (15/24, 62%). Small interstiti al deletions prevailed in BC and CC whereas large continuous and discontinu ous deletions were mainly found in RCC and NSCLC. Different epithelial tumo rs displayed unique LOH profiles with partial overlaps in 3p26.1, 3p21.31, and 3p13. The overlap around D3S2409 (3p21.31) appeared common for RCC, BC and CC. (C) 1999 Federation of European Biochemical Societies.