Investigations were carried out on experimental sausages, raw and steamed,
with the following levels of connective tissue: 1.8, 10, 20, 30, 50 and 76%
. Total protein content was determined by the Kjeldahl method, total collag
en by the modified Stegemann method, biological value by the PER method and
by the amino acid composition. The limiting amino acid (chemical score) wa
s also determined. It was found that an increase in the level of connective
tissue was followed by a significant decrease in PER and essential amino a
cid content, both in raw and steamed products. The decrease in particular a
mino acid contents was 42-89% (raw) and 38-92% (steamed). The smallest drop
was in the leucine level (38-43%), and the greatest in the tryptophan leve
l (89-92%). The limiting amino acid changed depending on the connective tis
sue content: at a low connective tissue content (1.8%), there were methioni
ne and cystine, at a medium content (10-20%) phenyloalanine and tyrozine, a
t a higher content (30:76%) tryptophan. Characteristic of steamed products
was a significantly higher biological value than in the raw products, which
was likely due to thermohydrolysis of collagen. The results indicate that
one of the criteria for quality meat products should be the quantitative le
vel of connective tissue.