Cadmium metallothionein gene of Tetrahymena pyriformis

A genomic sequence from Tetrahymena pyriformis, encoding a cadmium-induced metallothionein has been cloned. The gene encodes a transcript of 487 bases , with an intronless coding region of 324 nt, using TGA as the stop codon, TAA coding for glutamine, and the translational initiation sequence AAAATGG . Two regions of internal similarity in the coding sequence support the hyp othesis that the Tetrahymena protein arose by gene duplication. The sequenc es of untranslated regions show some similarities with nematode MT-I and MT -2 transcripts. Sequence of 525 bases upstream of the transcription start c ontains a TATA box, a CAAT box reverse complement, and many short stretches partially matching the AP-1 and ACE-I binding sites, but no characteristic sequences found in other metallothionein promoters. (C) 1999 Elsevier Scie nce B.V. All rights reserved.