Integration of cytogenetic with recombinational and physical maps of mousechromosome 16

To link the cytogenetic map for mouse chromosome 16 (Chr 16) to the more de tailed recombinational and physical maps, multiple probes were mapped by fl uorescence in situ hybridization (FISH). Sixteen large insert clones (YACs, BACs, and PACs) containing markers that have been assigned to the Chr 16 r ecombinational map were localized to a cytogenetic band or subband by high- resolution FISH. This linkage of the cytogenetic and recombinational maps p rovides a useful tool for assigning new probe locations and for defining br eakpoints in mice with chromosomal rearrangements. A direct application of these probes is demonstrated by identifying mice trisomic for distal Chr 16 using FISH analysis of interphase nuclei. (C) 1999 Academic Press.