Fs. Sharief et al., Cloning and chromosomal mapping of the human DNA polymerase theta (POLQ), the eighth human DNA polymerase, GENOMICS, 59(1), 1999, pp. 90-96
We have cloned the cDNA for the eighth human DNA polymerase, DNA polymerase
theta. The human cDNA encodes a putative DNA polymerase of 1762 amino acid
s with a calculated molecular mass of 198 kDa. The derived protein sequence
is homologous to the Drosophila melanogaster mus308 protein product, a put
ative DNA polymerase-helicase involved in repair of interstrand crosslinks.
The C-terminal region contains the canonical DNA polymerase motifs A, B, a
nd C found in the family A type of DNA polymerases, which includes Escheric
hia coli polymerase I. The N-terminal region contains a putative ATP bindin
g domain but not motifs for a helicase. The gene was mapped by radiation hy
brid analysis to chromosome 3q within an interval flanked by proximal marke
r D3S1303 and distal marker D3S3576 and, based on proximity to a gene that
has been mapped cylogenetically, within band 3q13.31. (C) 1999 Academic Pre
ss.