Differences in the concentrations df small, anionic, antimicrobial peptides in bronchoalveolar lavage fluid and in respiratory epithelia of patients with and without cystic fibrosis
Ka. Brogden et al., Differences in the concentrations df small, anionic, antimicrobial peptides in bronchoalveolar lavage fluid and in respiratory epithelia of patients with and without cystic fibrosis, INFEC IMMUN, 67(8), 1999, pp. 4256-4259
Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2
) antibodies to synthetic H-DDDDDDD-ON, an antimicrobial anionic peptide (A
P) originally isolated from ovine pulmonary surfactant, were prepared and u
sed to assess the concentrations of AP-like molecules in human respiratory
tract samples. In bronchoalveolar lavage fluids, concentrations of AP-like
molecules measured by enzyme-linked imnmunosorbent assay were significantly
lower in 13 patients with cystic fibrosis (CF) (mean +/- standard deviatio
n [SD], 0.78 +/- 0.46 mM) than in 34 patients without CF (1.30 +/- 0.66 mM)
(P = 0.01), In pulmonary tissues of three patients without CF, very little
antigen was stained in the apical cytoplasm of the bronchial and bronchiol
ar epithelium yet robust staining was seen in the alveolar epithelium. In p
ulmonary tissues of three patients with CF, robust staining of antigen was
seen in the apical cytoplasm of the bronchial and bronchiolar epithelium ye
t no staining was seen in the alveolar epithelium. These results show that
AP-like molecules are present in healthy human respiratory tract samples an
d differ in concentration and location of expression in patients with and w
ithout CF.