Expression and immunogenicity of a mutant diphtheria toxin molecule, CRM197, and its fragments in Salmonella typhi vaccine strain CVD 908-htrA

Authors
Orr, N Galen, JE Levine, MM
Citation
N. Orr et al., Expression and immunogenicity of a mutant diphtheria toxin molecule, CRM197, and its fragments in Salmonella typhi vaccine strain CVD 908-htrA, INFEC IMMUN, 67(8), 1999, pp. 4290-4294
Citations number
24
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
00199567 → ACNP
Volume
67
Issue
8
Year of publication
1999
Pages
4290 - 4294
Database
ISI
SICI code
0019-9567(199908)67:8<4290:EAIOAM>2.0.ZU;2-C
Abstract
Mutant diphtheria toxin molecule CRM197 and fragments thereof were expresse d in attenuated Salmonella typhi CVD 908-htrA, and the constructs were test ed for their ability to induce serum antitoxin. Initially, expressed protei ns were insoluble, and the constructs failed to induce neutralizing antitox in. Soluble CRM197 was expressed at low levels by utilizing the hemolysin A secretion system from Escherichia coil.