We developed a flow cytometric crossmatch (FC-CM) for the selection of
compatible donors for thrombocytopenic patients and compared its pred
ictive value for a successful platelet transfusion in a prospective st
udy to LCT. In case of alloimmunization, FC-CM detects bound IgG on ly
mphocytes and/or platelets after incubation with patient's serum, Base
d on the posttransfusion increment in 122 transfusions, the specifity
was 81% for LCT and 96% for FC-CM (platelet gate). In conclusion, the
FACS-CM is a rapid and useful assay for donor selection in cases of al
loimmunized patients with lymphocytotoxic and/or platelet-specific ant
ibodies.