Antiproliferative effects of the readily extractable fractions prepared from various Citrus juices on several cancer cell lines

To eliminate the masking effect by flavonoid glycosides, which comprise sim ilar to 70% of conventionally prepared sample, the readily extractable frac tion from Citrus juice, which was prepared by adsorbing on HP-20 resin and eluting with ethanol and acetone from the resin, was subjected to antiproli ferative tests against several cancer cell lines. Screening of 34 Citrus ju ices indicated that King (Citrus nobilis) strongly inhibited proliferation of all cancer cell lines examined. Sweet lime and Kabuchi inhibited three o f the four cancer cell lines. In contrast, these samples were substantially less cytotoxic toward normal human cell lines.