Extraction of polyphenolic compounds from grape seeds with near critical carbon dioxide

A new analytical method using near critical carbon dioxide to extract polyp henolic compounds from white grape seeds has been developed. Carbon dioxide density, organic modifier, percentage of modifier, and extraction temperat ure were optimized utilizing an experimental design. Gallic acid, catechin, and epicatechin were the main phenolic compounds detected in the HPLC chro matogram of each extract. Recovery and reproducibility of catechin from gra pe seed was calculated. Under optimized conditions recovery was estimated t o be 79% with a RSD equal to 7.3%. Results from the supercritical fluid met hod were compared with results obtained via liquid-solid extraction using m ethanol-water. (C) 1999 Elsevier Science B.V. All rights reserved.