Determination of catecholamines in human plasma by high-performance liquidchromatography with electrochemical detection

In the present study, assays were improved for the determination of catecho lamines in human plasma. High-performance liquid chromatography with electr ochemical detection was employed for quantitative analysis. The influence o f various parameters on chromatographic performance, such as the compositio n and the pH of the mobile phase, and the detection potential, was investig ated. An accurate solid-phase extraction procedure, after catecholamine com plexation with diphenylborate, was developed. The efficiency yield for all catecholamines was in the range 92-98%. Relative standard deviation values for repeatability and for intermediate precision were less than 2% and 3%, respectively, for all three analytes. (C) 1999 Elsevier Science B.V. All ri ghts reserved.