Sd. Reid et al., Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR, J CLIN MICR, 37(8), 1999, pp. 2719-2722
A multiplex PCR was designed to detect the eae gene and simultaneously iden
tify specific alleles in pathogenic Escherichia coli. The method was tested
on 87 strains representing the diarrheagenie E. coli clones. The results s
how that the PCR assay accurately detects eae and resolves alleles encoding
the alpha, beta, and gamma intimin variants.