Novel immunoblot assay using four recombinant antigens for diagnosis of Epstein-Barr virus primary infection and reactivation

Authors
Buisson, M Fleurent, B Mak, M Morand, P Chan, L Ng, A Guan, M Chin, D Seigneurin, JM
Citation
M. Buisson et al., Novel immunoblot assay using four recombinant antigens for diagnosis of Epstein-Barr virus primary infection and reactivation, J CLIN MICR, 37(8), 1999, pp. 2709-2714
Citations number
20
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
8
Year of publication
1999
Pages
2709 - 2714
Database
ISI
SICI code
0095-1137(199908)37:8<2709:NIAUFR>2.0.ZU;2-B
Abstract
A new immunoblot assay, composed of four Epstein-Barr virus (EBV)-encoded r ecombinant proteins (virus capsid antigen [VCA] p23, early antigen [EA] p13 8, E4 p54, and EBNA-1 p72), was compared with an immunofluorescence assay o n a total of 291 sera. The test was accurate in 94.5% of cases of primary E BV infection, while an immunoglobulin G anti-VCA p23 band with strong inten sity correlated with reactivation.