Biochemical and crystallographic characterization of a Rho effector domainof the protein serine threonine kinase N in a complex with RhoA

Authors
Maesaki, R Shimizu, T Ihara, K Kuroda, S Kaibuchi, K Hakoshima, T
Citation
R. Maesaki et al., Biochemical and crystallographic characterization of a Rho effector domainof the protein serine threonine kinase N in a complex with RhoA, J STRUCT B, 126(2), 1999, pp. 166-170
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STRUCTURAL BIOLOGY
ISSN journal
10478477 → ACNP
Volume
126
Issue
2
Year of publication
1999
Pages
166 - 170
Database
ISI
SICI code
1047-8477(19990615)126:2<166:BACCOA>2.0.ZU;2-B
Abstract
The effector domain of human protein serine/threonine kinase N (PKN), an ef fector protein for the small GTP-binding protein Rho, was expressed and pur ified for protein characterization and crystallization in a complex form wi th human RhoA. In solution, RhoA binds to the PKN effector domain with 1:2 stoichiometry in a GTP-dependent manner. The obtained complex crystals diff ract to 2.2 Angstrom resolution. (C) 1999 Academic Press.