Isolation of antioxidative compounds from bamboo shoots sheath

Antioxidative activity of constituents from ethyl acetate extract of bamboo shoots sheath was studied by the following procedures. The extracts were s ilica gel column chromatographed using the solvent system of hexane-ethyl a cetate-methanol and moreover, antioxidative fractions were purified by ODS- HPLC using the elution solvent system of H2O-methanol. Antioxidative activi ty of thus obtained fractions were examined by the POV method. As the resul ts, two antioxidative constituents of I and II were isolated and identified as Tricin and Taxifolin for the first time, respectively. The antioxidativ e activities of I and II were about 10% and 1% of that of alpha-Tocopherol, respectively.