Flow injection analysis of gamma-aminobutyric acid in GABA-enriched tea

Authors
Horie, H Sawai, Y Kohata, K
Citation
H. Horie et al., Flow injection analysis of gamma-aminobutyric acid in GABA-enriched tea, J JPN SOC F, 46(7), 1999, pp. 494-496
Citations number
1
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
ISSN journal
1341027X → ACNP
Volume
46
Issue
7
Year of publication
1999
Pages
494 - 496
Database
ISI
SICI code
1341-027X(1999)46:7<494:FIAOGA>2.0.ZU;2-4
Abstract
We have developed a flow injection method to determine gamma-aminobutyric a cid (GABA) which is known as the main component for suppression of blood pr essure in GABA-enriched tea. The concentration of GABA was measured by the fluorescence of NADPH produced in an immobilized GABAse reactor. The interf erence originated from the fluorescence of tea components was diminished by acidifying the mobile phase and passing it through an ODS-minicolumn befor e fluorescent detection. The GABA content of GABA-enriched teas measured by this method highly correlated with that measured by the standard HPLC meth od.