J. Charlwood et al., Structural characterisation of N-linked glycan mixtures by precursor ion scanning and tandem mass spectrometric analysis, RAP C MASS, 13(14), 1999, pp. 1522-1530
2-Aminoacridone (2-AMAC) labelled N-linked glycan pools were analysed direc
tly by a hybrid quadrupole orthogonal acceleration time-of-flight mass spec
trometer (Q-Tof) in the precursor ion scanning and tandem mass spectrometry
(MS/MS) modes, The use of a precursor ion scanning strategy on this instru
ment provides a rapid and sensitive method of screening glycan mixtures, wi
thout prior separation by chromatographic methods, It allows facile and pre
liminary characterisation of glycans into different classes, for example, h
igh-mannose or complex glycans, Preliminary sequencing information for each
glycan is obtained in the initial precursor ion scanning mode, but further
sequencing information of selected glycans can be obtained using the MS/MS
mode. Copyright (C) 1999 John Whey & Sons, Ltd.