Patients with haematological disorders (n=100) were examined for prevalence
of parvovirus B19 DNA in the bone marrow and serum, irrespective of B19-re
lated symptoms. B19 DNA was studied using 2 nested PCRs and the serum sampl
es were further analysed with B19-specific IgG, IgM and avidity as well as
seroreactivity against linear and conformational epitopes of the B19 VP2 an
tigen. The Latter assays specify whether the IgG antibody response represen
ts acute or past B19 infection. B19 DNA was detected in 4 of the 100 bone m
arrow samples, whereas all the serum samples were B19 DNA negative. None of
the 4 B19 DNA positive patients had symptoms typical of B19 infection and
serology showed past infection. Furthermore, 2 were still B19 DNA positive
in bone marrow more than 1 y after the first sample indicating virus persis
tence. The seroprevalence for B19 IgG was 59% and 2 patients mere B19 IgM p
ositive. Thus, presence of B19 DNA in bone marrow from patients with haemat
ological disorders is not a general finding in seropositive patients. B19 D
NA can persist in bone marrow, but in our material this finding showed no c
lear correlation with symptomatic B19 infection.