Determination of sparfloxacin in plasma and urine by a simple and rapid liquid chromatographic method

A simple, specific, sensitive, and rapid method has been developed and vali dated for the determination of sparfloxacin in human plasma and urine. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma p roteins were efficiently removed by precipitation with perchloric acid afte r the addition of grepafloxacin as an internal standard, For the urine samp les, the only required sample preparation was dilution. Separation was achi eved on a C-18 reversed-phase column. The quantification limit was 0.025 mg /L in plasma and 0.5 mg/L in urine. The coefficients of variation (CV) were less than 10% for intra-day and inter-day analyses. The recovery of sparfl oxacin added to plasma and urine ranged from 96.7% to 97.9%. The method has been successfully applied to pharmacokinetic studies.