Analysis of cell surface properties using derivatized agarose beads

An assay has been developed to analyse cell surface properties using agaros e beads derivatized with amino acids, sugars, proteins, and other molecules . The assay is simple and rapid and is useful to identify new cell surface markers. Various species and strains of yeast, paramecium, and Euglena were tested for their ability to bind to over 100 types of derivatized beads. A variety of specificity studies were performed in order to understand the n ature of cell-bead binding. Our results indicate that cell-bead binding is often specific enough to distinguish between configurational isomers and sp acer sizes and can be blocked by addition of specific molecules to the incu bation medium. In some cases, different species or strains differed only by their binding to a single bead type. This simple and rapid assay may help to uncover new cell surface receptors and may lead to the development of cl inically useful compounds for therapeutic applications.