Differential display analysis of gene expression accompanied by neurite outgrowth of human neuroblastoma Cell IMR32 using non-gel molecular sieving capillary electrophoresis
N. Ishioka et al., Differential display analysis of gene expression accompanied by neurite outgrowth of human neuroblastoma Cell IMR32 using non-gel molecular sieving capillary electrophoresis, ANALUSIS, 27(2), 1999, pp. 166-169
The induced proliferative response of the human derived neuroblastoma cell
(IMR32), with the cell proliferation mediating factor, is much milder compa
red to other cell strains, and develops cellular clusters which are charact
erized by numerous extensions of dendrites. Presuming this phenomenon to be
one of the induced neuronal differentiation due to genetic alterations, we
preliminary studied to elucidate the changes in gene expression of the IMR
32 by mRNA differential display analysis, monitoring polymerase chain react
ion (PCR) products by non-gel molecular sieving capillary electrophoresis i
n linear polyacrylamide solution. The CE fingerprints revealed a number of
peaks with differential expression patterns.