L. Bromberg et L. Salvati, Bioactive surfaces via immobilization of self-assembling polymers onto hydrophobic materials, BIOCONJ CHE, 10(4), 1999, pp. 678-686
Conjugation of proteins to copolymers from poly(acrylic acid) grafted onto
PEO-PPO-PEO backbone (Pluronic-PAA) following adsorption of the conjugates
onto hydrophobic surfaces is reported. Insulin-Pluronic-PAA conjugates show
negligible internalization of insulin into human uterine smooth muscle cel
ls as well as enhancement of mitogenic activity. Glucose-induced release of
glycated albumin complexed with a Pluronic-PAA-concanavalin conjugate and
adsorbed onto polystyrene nanospheres may provide a model for a glucose-res
ponsive protein delivery system or a heterogeneous diagnostic device.