Two-state irreversible thermal denaturation of muscle creatine kinase

Thermal denaturation of creatine kinase from rabbit skeletal muscle has bee n studied by differential scanning calorimetry. The excess heat capacity vs . temperature profiles were independent of protein concentration, but stron gly temperature scanning rate-dependent. It has been shown that thermal den aturation of creatine kinase satisfies the previously proposed validity cri teria for the two-state irreversible model [Kurganov et al., Biophys. Chem. 70 (1997) 125]. The energy activation value has been calculated to be 461.0 +/- 0.7 kJ/mol. (C) 1999 Elsevier Science B.V. All rights reserved.