T. Mynster et al., Prestorage and bedside leucofiltration of whole blood modulates storage-time-dependent suppression of in vitro TNF alpha release, BR J HAEM, 106(1), 1999, pp. 248-251
Immunosuppression after transfusion may be related to the content of leucoc
ytes in the transfused blood. Therefore we studied the effects of prestorag
e and bedside leucodepletion by filtration on the suppression by whole bloo
d of in vitro stimulated tumour necrosis factor alpha (TNF alpha) release,
Nine units of whole blood were leucofiltered prestorage and stored for 35 d
, 27 units, 3 x 9, were stored and leucofiltered at the bedside after 7, 21
and 35d, Supernatants were collected from all units during storage and add
ed to a whole blood assay of E. coli-LPS-stimulated TNF alpha release. The
effects of storage were assessed and compared with supernatants collected i
mmediately after donation as reference. TNF alpha release was storage time
dependently suppressed to: 81%, 74% and 57% by supernatants from non-filter
ed blood stored for 7, 21 and 35d, respectively. Prestorage leucofiltration
almost eliminated this effect, but we still observed a storage-time-depend
ent suppression by bedside-leucofiltered blood to 88%, 78% and 65%, respect
ively Prestorage leucofiltration appeared to reduce storage-time-dependent
suppression of in vitro stimulated TNF alpha release induced by plasma from
whole blood compared with non-filtered and bedside-leucofiltered whole blo
od.