W. Golkiewicz et M. Gadzikowska, Isolation of some quaternary alkaloids from the extract of roots of Chelidonium majus L. by column and thin-layer chromatography, CHROMATOGR, 50(1-2), 1999, pp. 52-56
A chromatographic system comprising untreated silica gel and an aqueous buf
fer - methanol eluent was investigated for the micropreparative separation
of the alkaloids of Chelidonium majus L. The relationship between the logar
ithm of the retention factors k of the alkaloids and volume fraction of met
hanol at pH* 6 was linear for volume fractions in the range of 0.1 to 0.4.
From this relationship it is possible to estimate the value of the retentio
n factor for a given alkaloid in pure buffer and then the maximum volume of
alkaloid extract that can be sampled on micropreparative column filled wit
h silica gel.