Reversed-phase separations on cellulose tris(3,5-dimethyl phenylcarbamate)chiral stationary phase

The chiral separation of eight racemic compounds has been investigated on a cellulose tris(3,5-dimethylphenylcarbamate) chiral column under reversed-p hase conditions. The discrimination mechanism under reversed-phase conditio ns is discussed. Addition of acid to the mobile phase is necessary for reso lution of acidic racemic compounds. The presence of ion-pair reagent in the mobile phase is a key factor in the resolution of basic racemic compounds. Retention of the racemates is also affected by addition of acid or salt. T he anion in buffer not only interacts wit the racemate, but also with the p olysaccharide derivative on the silica gel surface.