Diphasic dialysis extraction technique for clenbuterol determination in bovine retina by gas chromatography - Mass spectrometry

A method is described for the extraction of clenbuterol from bovine retina samples by diphasic dialysis using gas chromatography - mass spectrometry ( GC-MS) as the procedure of determination. Following chemical digestion of r etinal tissue in alkaline medium at 80 degrees C, clenbuterol is extracted by diphasic dialysis using diethyl ether as solvent at 37 degrees C, for 4 h at 150 rpm. Diethyl ether is evaporated to dryness and clenbuterol determ ined by GC-MS after butylboronic acid (BBA) derivatization. A detection lim it of 2.5 ng g(-1), repeatability of 9.0% and reproducibility of 22.9%, wit h recoveries always > 86% (n=5), are achieved as validation criteria.