APPLICATION OF A MODIFIED CENTRAL COMPOSITE DESIGN TO OPTIMIZE THE CAPILLARY ELECTROCHROMATOGRAPHIC SEPARATION OF RELATED S-OXIDATION COMPOUNDS

Capillary electrochromatography was employed to separate the antibacte rial 3-[4-(methylsulfinyl) phenyl]-5S-acetamidomethyl-2-oxazolidinone from its related S-oxidation products. The separation was optimized by a systematic search for the optimal separation conditions employing a modified central composite design. The variables examined in the opti mization were in terms of applied potential, volume fraction of aceton itrile and buffer (Tris hydrochloride) concentration. A response surfa ce mapping for selected variables at an applied potential of 25 kV was also performed.