SM37, a skeletogenic gene of the sea urchin embryo linked to the SM50 gene

Citation
Yh. Lee et al., SM37, a skeletogenic gene of the sea urchin embryo linked to the SM50 gene, DEVELOP GR, 41(3), 1999, pp. 303-312
Citations number
28
Categorie Soggetti
Cell & Developmental Biology
Journal title
DEVELOPMENT GROWTH & DIFFERENTIATION
ISSN journal
00121592 → ACNP
Volume
41
Issue
3
Year of publication
1999
Pages
303 - 312
Database
ISI
SICI code
0012-1592(199906)41:3<303:SASGOT>2.0.ZU;2-5
Abstract
The genomic DNA region that contains the SM50 gene also included a second g ene that appeared to encode another skeletogenic matrix protein. The two ge nes were linked at a distance of about 12 kb. Based on the molecular weight of the implied protein the gene was termed SM37. The SM37 protein included a long tandem sequence of short glycine-rich repeats that was similar to t he glycine-rich repeats included in the SM50 protein and several other skel etal matrix proteins, although the sequence of SM37 repeat was distinct. Th e overall structure of the SM37 protein was similar to SM50 as well. Howeve r, SM37 was only about 30% identical in amino acid sequence to SM50, and co ding region probes behaved as a single copy sequence under standard conditi ons. The SM37 gene included the same cia-regulatory elements as the SM50 ge ne, although in a different order with respect to transcription. This gene was regulated coordinately with SM50 during development, and like SM50 was expressed exclusively in skeletogenic mesenchyme lineages.