Papanicolaou (Pap) tests reported as CIN I (cervical intraepithelial neopla
sia, grade 1) may be subject to laboratory misclassification because of scr
eening and interpretative errors. A peer-group consensus review was conduct
ed to measure the misclassification rate of Pap rests reported as CIN I and
to analyze the undercalled and overcalled tests for due cause. Four hundre
d and forty-nine Pap rests originally reported as CIN I were independently
reviewed twice by a panel of four pathologists, and disagreements were reso
lved by consensus review. Results were based on the original screening for
the first review and following the removal of those markings, were based on
a second independent rescreening for the second review. A review result of
low-grade squamous intraepithelial lesion (LSIL) and atypical squamous cel
ls of undetermined significance (ASCUS)Savoring LSIL was equated with the o
riginal CIN I result. Final classification was based on the second consensu
s review. Misclassified tests were categorized as screening or interpretati
ve errors, based on a comparison of the review classifications. LSIL and AS
CUS Savoring LSIL were repel-led in 85.1% and 73.9% of the first and second
reviews, respectively. In the final classification there were 362 (80.6%)
LSIL and ASCUS-LSIL and 87 (19.4%) misclassifications: 31 (6.9%) undercalls
and 56 (12.5%) overcalls. Screening error accounted for 35.5% of undercall
ed tests, and the remainder were interpretative errors, as were all those o
vercalled. In this study Pap tests reported as CIN I were subject to miscla
ssification because of a laboratory error in 19.4% of tests. Reductions in
screening and interpretative errors were identified as mechanisms for impro
ving accuracy. Diagn. Cytopathol, 1999;21:129-136. (C) 1999 Wiley-Liss, Inc
.