LIQUID-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF BECLICONAZOLEAND ITS POTENTIAL IMPURITIES

High-performance liquid chromatography (HPLC) was employed for the sep aration and determination of becliconazole, an imidazole derivative wi th antifungal activity containing a stereogenic centre, and its impuri ties. Chiral columns, containing carbamate of cellulose and amylose, w ere used, The effects of the organic modifiers, ethanol and 2-propanol , in the mobile phase, were studied. The HPLC method gave good perform ances from qualitative and quantitative standpoints, allowing the enan tiomeric ratio of becliconazole and its impurities to be determined. T he assay showed linearity over the range 400-4000 ng ml(-1) for the im purities and 400-4000 mu g ml(-1) for becliconazole.