Validation of an enzymatic method for the determination of trace levels ofethanol

Authors
Alvencar, RV Lam, S Sidhom, M Okarter, TU
Citation
Rv. Alvencar et al., Validation of an enzymatic method for the determination of trace levels ofethanol, DRUG DEV IN, 25(8), 1999, pp. 929-935
Citations number
21
Categorie Soggetti
Pharmacology & Toxicology
Journal title
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
ISSN journal
03639045 → ACNP
Volume
25
Issue
8
Year of publication
1999
Pages
929 - 935
Database
ISI
SICI code
0363-9045(1999)25:8<929:VOAEMF>2.0.ZU;2-1
Abstract
The validation of an enzymatic method for the determination of trace levels of ethanol is described. The method, which involves the use of alcohol deh ydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD(+)), was valida ted according to USP 23 requirements. The validation parameters, including linearity and range, accuracy (recovery), precision, ruggedness, limit of d etection (LOD), and limit of quantitation (LOQ), were established satisfact orily. The method is specific and selective for ethanol except in the prese nce of propyl, isopropyl, or butyl alcohol.